V vs [S] KINETICS (Michaelis-Menten)
Normal (Uninhibited)
Vmax: 100
Competitive
Vmax Unchanged | Km ↑
Non-competitive
Vmax ↓ | Km Unchanged
LINEWEAVER-BURK
Double Reciprocal Plot
y-intercept = 1 / Vmax
x-intercept = -1 / Km
y-intercept = 1 / Vmax
x-intercept = -1 / Km
0100
Michaelis-Menten Equation:
■ Competitive Inhibition:
Inhibitor binds active site. Can be overcome by adding more substrate. Vmax remains unchanged. Km increases.
■ Non-competitive (Allosteric):
Inhibitor binds elsewhere, altering enzyme shape. Cannot be overcome by [S]. Km remains unchanged. Vmax drops.
V = (Vmax * [S]) / (Km + [S])■ Competitive Inhibition:
Inhibitor binds active site. Can be overcome by adding more substrate. Vmax remains unchanged. Km increases.
Km' = Km * (1 + [I]/Ki)■ Non-competitive (Allosteric):
Inhibitor binds elsewhere, altering enzyme shape. Cannot be overcome by [S]. Km remains unchanged. Vmax drops.
Vmax' = Vmax / (1 + [I]/Ki)